Effect of Human Mesenchymal Stem Cell-Conditioned Medium Injection on Oxidative Stress Induced by Carbon Tetrachloride in the Liver Tissue of Rats

Background: Carbon tetrachloride (CCI4) is used as a chemical intermediate in industries. It can be converted into toxic reactive products of trichloromethyl radical under the influence of cytochrome P450 enzymes, and cause tissue damage, including liver damage, through oxidative stress. Liver transplantation is an effective treatment for liver failure but is limited due to the shortage of organ donors. Objective: This study aims to evaluate the effect of human mesenchymal stem cell-conditioned medium (hMSC-CM) on the oxidative stress induced by CCI4 in rats. Methods: Twenty-one adult male Wistar rats matched for weight were randomly allocated into three groups of 7: control, treatment A (receiving CCI4), and treatment B (receiving CCI4 + hMSC-CM). A single dose of normal saline was injected into the rats in the control group. Treatment group A and B received intraperitoneal injection of CCI4 along with olive oil with a ratio of 1:1. Twenty-four hours after CCI4 injection, the rats in group B were treated by injection of hMSC-CM for three consecutive days. Forty-eight hours after the injection of hMSC-CM, blood samples were collected from all rats. Oxidative stress markers including total antioxidant capacity (TAC) and malondialdehyde (MDA) were measured and the data were analyzed using one-way analysis of variance in SPSS software. Results: There was no statistically significant difference in TAC and MDA levels between different groups (P>0.05). The highest levels of TAC was observed in treatment group B, and the highest level of MDA was observed in treatment group A. Conclusion: The hMSC-CM has no significant effect on the oxidative stress in the liver. It is possible to achieve significant results by increasing the duration of treatment.